Association of platelet endothelial cell adhesion molecule-1 gene polymorphism (Leu125Val) with coronary artery disease in type II diabetics and nondiabetics

El-Kishki, Maha M.S.; Abdelfattah Alramly, Amal M.; Taha, Mohamed O.; El Din Medhat, Rana A.

doi:10.4103/sjamf.sjamf_41_18

Association of platelet endothelial cell adhesion molecule-1 gene polymorphism (Leu125Val) with coronary artery disease in type II diabetics and nondiabetics

Authors

Abstract

Background and objectives
Platelet endothelial cell adhesion molecule-1 (PECAM-1) plays a key role in the transendothelial migration of circulating leukocytes (diapedesis) during vascular inflammation. Polymorphism (Leu125Val) of the (373C/G) is functional. It was reported to be associated with high serum level of PECAM-1. We hypothesized that this genetic variation of the could be associated with the development of atherosclerosis. Therefore we conducted a study to investigate the association between single-nucleotide polymorphism of the , C+373G (Leu125Val) at exon 3, in Egyptian patients with coronary artery disease.
Patients and methods
Blood samples were withdrawn from 40 coronary artery disease patients and 20 age-matched and sex-matched controls. The single-nucleotide polymorphism of the was analyzed by PCR-restriction fragment length polymorphism strategy.
Results
Genotype distributions between patient and control groups showed no significant statistical difference regarding the CC genotype, where 22.5% of patients and 35% of controls carried this genotype (=0.470). As for the CG genotype, a statistically significant higher CG genotype distribution was found in patients, where 52.5% of patients and only 20% of controls carried this genotype (=0.033). There was no statistically significant difference in GG distributions between patient and control groups, where 25% of patients and 45% of controls carried this genotype (=0.202). No significant statistical difference was observed in allele frequency between the two groups, where 51.25% of patients and 55% of controls carry the G allele and 48.7% of patients and 45% of controls carry the C allele (=0.846).
Interpretation and conclusion
We concluded that our study demonstrated a possible effect of PECAM-1 (Leu125Val) polymorphism on the development of atherosclerosis.

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